BSc-1 Year Biotechnology syllabus 

In university B.Sc.-I year The syllabus of BIOTECHNOLOGY consists of two papers of theory and one paper of practical's.

PAPER – I

BIOCHEMISTRY, BIOSTASTICS AND COMPUTERS

UNIT-I

1. Introduction to Biochemistry: History, Scope and Development.

2. Carbohydrates: Classification, Structure and Function of Mono, Oligo and Polysaccharides.

3. Lipids: Structure, Classification and Function.

UNIT –II

1. Amino acids and Proteins: Classification, Structure and Properties of amino acids, Types of Proteins and their Classification and Function.

2. Enzymes: Nomenclature and Classification of enzyme, Mechanism of enzyme action, Enzyme Kinetics and Factors affecting the enzymes action. Immobilization of enzyme and their application.

UNIT –III

1. Hormones: Plant Hormone-Auxin and Gibberellins and Animal Hormone-Pancreas and Thyroid.

2. Carbohydrates, Proteins and Lipid Metabolism - Glycolysis, Glycogenesis,

Glyconeogenesis, Glycogenolysis and Krebs cycle. Electron Transport Chain and β[1]oxidation of Fatty acids.

UNIT-IV

1. Scope of Biostatistics, Samples and Population concept, Collection of data-sampling techniques, Processing and Presentation of data.

2. Measures of Central Tendency: Mean, Median and Mode and Standard Deviation.

3. Probability Calculation: Definition of probability, Theorem on total and compound probability.

UNIT-V

1. Computers - General introduction, Organization of computer, Digital and Analogue

Computers and Computer Algorithm.

2. Concept of Hardware and Software, Input and Output Devices.

3. Application of computer in co-ordination of solute concentration, pH and Temperature etc., of a Fermenter in operation and Internet application

 

B.Sc.-I year BIOTECHNOLOGY

PAPER–II

CELL BIOLOGY, GENETICS AND MICROBIOLOGY

UNIT-I

1. Concept of life, Cell as a basic unit of living system and Cell theory.

2. Diversity of Cell shape and size.

3. Prokaryotic cell structure: Function and ultra structure of cell (Gram positive and Gram negative Bacteria), Plasma membrane, Flagella, Pilli, Endospore and Capsule.

4. Eukaryotic cell: Plant cell wall and Plasma membrane.

UNIT-II

1. Cytoplasm: Structure and Functions of Endoplasmic reticulum, Ribosome, Golgi

complex, Lysosomes, Nucleus, Mitochondria and Chloroplast.

2. Cytoskeleton: Microtubules, Microfilaments and Intermediate filaments.

3. Cell division: Mitosis and Meiosis.

4. Programmed Cell Death..

UNIT-III

1. Mendel’s Laws of Inheritance.

2. Linkage and Crossing over.

3. Chromosome variation in number and structure: Deletion, Duplication, Translocation, Inversion and Aneuploidy, Euploidy (Monoploidy and Polyploidy and its importance).

UNIT-IV

1. History, Scope and Development of Microbiology.

2. Basic techniques of Microbial Culture

3. Microbial Growth & Nutrition of Bacteria: Isolation, media sterilization- physical and chemical agents, pure culture-pour plate method, streak plate method and spread plate method.

4. General features and Economic importance of Fungi, Algae and Protozoa etc.

UNIT-V

1. Bacterial Reproduction: Conjugation, Transduction and Transformation.

2. Mycoplasma – History, Classification, Structure reproduction & Diseases.

3. Viruses – Basic features, Structure, Classification, Multiplication, Bacteriophages

 (Morphology, life cycle, infection and medicinal importance)

      

List of Practical’s

MICROBIOLOGY AND BIOCHEMICAL TECHNIQUES

(1) Laboratory rules, Tools, Equipment and Other requirements in Microbiological laboratory.

(2) Micrometry – Use of ocular & stage Micrometrer.

(3) Counting of bacteria by counting chamber, by plate count.

(4)Preparation of media and cultivation techniques:

• (a) Basic liquid media (broth)
• (b) Basic Solid media, (agar slants and deep tubes)
• (c) Demonstration of selective and differential media
• (d) Isolation and enumeration of micro organisms
• (e) Isolation from air and Soil

(5)Smears and staining methods:

• (a) Preparation of bacterial smear
• (b) Gram Negative & Positive staining

(6)Methods of obtaining pure cultures

• (a) Streak plate method
• (b) Pure plate method
• (c) Spread plate method
• (d) Broth cultures

(7)Growth & Biochemical techniques

• (a) Determination of bacterial growth curve
• (b) Amylase production test
• (c) Cellulose production test
• (d) Estimation of Sugar in given solution
• (e) Extraction and separation of lipids
• (f) Estimation of proteins
• (h) Mitosis and Meiosis

(8)Biostatistics:

• (a) By Manual and by computer.
• (b) Problems on mean, mode and median

 

 

SCHEME OF PRACTICAL EXAMINATION

Time – 4hrs. M. M.: 50

• 1. Experiment based on culture of micro-organisms 15 Marks
• 2. Bacterial growth/Staining techniques 10 Marks
• 3. Biochemical techniques 05 Marks
• 4. Bio statistics 05 Marks
• 5. Spotting 05Marks
• 6. Viva – Voce 05Marks
• 7. Record/Sessional 05 Mark